DNA on the move – the influence of form on gel migration
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DNA on the move – the influence of form on gel migration
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Agarose gel electrophoresis is a staple method in almost all biology and biochemistry lab where separation and analysis of nucleic acids is needed. In this Mol Bio Minutes mini episode Augustė Užuotaitė, Scientist III at Thermo Fisher Scientific, covers the basics of electrophoresis with a spotlight on how different forms of DNA migrate differently in agarose gel electrophoresis.
In less than 10 quick minutes, you’ll learn about the many factors that affect DNA migration rate. Augustė reviews how DNA size, sequence, and conformation all affect migration rate, and she gives some beautifully simple examples to help it all make sense.
Helpful resource links mentioned in this episode:
- Nucleic acid gel electrophoresis – summarized in 5 easy steps
- Five important considerations for the nucleic acid gel electrophoresis
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For Research Use Only. Not for use in diagnostic procedures.